Fluorescence microscope refers to any microscope that uses fluorescence to generate an image, whether it is a more simple set up like an epifluorescence microscope or a more complicated design such as a confocal microscope, which uses optical sectioning to get better resolution of the fluorescence image. What is the abbreviation for episcopic fluorescence image capture. Cardiac muscle organization revealed in 3d by imaging whole. Software application to capture and display single images, image sequences and image data streams from all wdm video sources cameras, converters etc. Any or all of the following could influence your choice of platform. Yap, choon hwai, pekkan, kerem, and lo, ceilia wen ya. Development of the knee joint was morphologically investigated, and the process of cavitation was analyzed by using episcopic. Episcopic fluorescence image capture efic and high resolution episcopic microscopy hrem are blockface imaging techniques that acquire superior resolution. Weve got 1 shorthand for episcopic fluorescence image capture. With this imaging technique weninger and mohun, 2002, the embryo is embedded in paraffin. The tutorial initializes with a cutaway schematic diagram of a reflected light fluorescence microscope appearing in the window.
Microcomputed tomography provides high accuracy congenital. We describe a detailed protocol using highresolution episcopic microscopy to. Spatial change of cruciate ligaments in rat embryo knee joint by. Then using a sliding microtome, the block is sectioned and the block face is photographed with each. The userfriendly interface, complete camera parameter control, advanced image processing functions, live and still image measurements provide for highly efficient workflow. In most cases, the emitted light has a longer wavelength, and therefore lower energy, than the absorbed radiation. Tcapture is the professional imaging software application developed by tucsen for full control of tucsen cameras. Rapid high resolution three dimensional reconstruction of embryos with episcopic fluorescence image capture. Of the techniques, episcopic fluorescence image capture efic12, and highresolution episcopic microscopy 14 have been successfully applied in recent research, while other applications, such as fast 3d serial reconstruction 15, surface imaging microscopy16, 17, and serial blockface scanning electron microscopy 18, are not yet routine and. Some codecs will be unavailable in the 64bit version. Yet another novel method that can provide registered 2d image stacks suitable for rapid 3d rendering is episcopic fluorescence image capture efic. Excitation light travels along the illuminator perpendicular to the optical axis of the microscope, passes through collector lenses and a variable, centerable aperture diaphragm, and then through a variable, centerable field diaphragm see. A custom automated episcopic fluorescent image capture device. Secondly, episcopic fluorescence image capture efic and phasecontrast xray computed.
Rapid high resolution three dimensional reconstruction of. Of the techniques, episcopic fluorescence image capture efic12, and high resolution episcopic microscopy have been successfully applied in recent. Of the techniques, episcopic fluorescence image capture efic12, and highresolution episcopic microscopy have been successfully applied in recent. Olympus microscopy resource center fluorescence microscopy. Download scientific diagram a custom automated episcopic fluorescent image capture device right that is optimized to image the surface of the embedded. Using episcopic fluorescence image capture, ultrasound biomicroscopy and computational fluid dynamics to study geometry and fluid mechanics of mouse fetus and neonate. Introduction to fluorescence microscopy nikons microscopyu. Threedimensional reconstruction of rat knee joint using episcopic. Looking for the shorthand of episcopic fluorescence image capture. This page is about the various possible meanings of the acronym, abbreviation, shorthand or slang term. Aug 28, 2014 episcopic fluorescence image capture efic was performed on 66 wildtype mouse embryos from embryonic day e 9. Objectivedevelopment of the knee joint was morphologically investigated, and the process of cavitation was analyzed by using episcopic fluorescence image capture efic to create spatial and temporal threedimensional 3d reconstructions. A microscopic imaging technique that serially sections embedded biological specimens and.
In reflected light kohler illumination for fluorescence microscopy, an image of the light source is focused by the collector lens onto the aperture iris diaphragm located in the vertical illuminator. Human breast duct anatomy, the sick lobe hypothesis and. In order to operate the tutorial, use the field diaphragm and aperture diaphragm sliders to adjust the field size and numerical aperture. Reflected episcopic light illumination nikons microscopyu. Episcopic fluorescence image capture efic was performed on 66 wildtype mouse embryos from embryonic day e 9. Mar 26, 20 a wide spectrum of chd was detected by the ubm, which were subsequently confirmed with followup necropsy and histopathology examination with episcopic fluorescence image capture. Rapid acquisition of 3d images using highresolution episcopic. These allow analysis of molecular signals in a wide variety of biological samples such as tissues or embryos, in their precise anatomical and histological context. Digital image capture systems require many of the same considerations as film systems, although white balance adjustment on the imaging device digital camera allows considerable latitude in matching camera response to the color characteristics of various light sources. Fluorescence illumination and observation is the most rapidly expanding microscopy technique employed today, both in the medical and biological sciences, a fact which has spurred the development of more sophisticated microscopes and numerous fluorescence accessories. Rapid 3d phenotyping of cardiovascular development in mouse. Episcopic fluorescence image capture, on the other hand, is a promising new histological technique that can generate a 3d data set from sectioned material. Design and implementation of a custom built optical.
We provide simple protocols for generating digital volume data with the highresolution episcopic microscopy hrem method. Threedimensional analysis of molecular signals with. Automation of a relatively rapid image capture cycle and the ability to choose. Confocal enhanced episcopic fluorescent image capture efic. Ultrahigh frequency ultrasound biomicroscopy and high.
A solution to this problem is a novel imaging modality for the generation of highresolution 3d reconstructed images 61, which uses episcopic fluorescence image capture efic. To meet this need, several 3d fluorescent imaging techniques have been. Hrem is capable of imaging organic materials with volumes up to 5 x 5 x 7 mm 3 in typical numeric resolutions between 1 x 1 x 1 and 5 x 5 x 5 m 3. Episcopic fluorescence image capture is widely applied for experimental animals, congenital anomalies on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. This chapter describes two episcopic imaging methods, episcopic fluorescence image capturing efic and highresolution episcopic microscopy hrem. The symphotime 64 software package is an integrated solution for data acquisition and analysis using picoquants timeresolved confocal microscope microtime 200, microtime 100, lsm upgrade kits or tcspc electronics. Cruciate ligaments of wister rat embryos between embryonic day e 16 and e20 were analyzed. Episcopic fluorescence image capture efic and highresolution episcopic. As presented in figure 1, the reflected light vertical illuminator comprises an arcdischarge lamphouse at the rear end usually a mercury or xenon burner. The 2d image stacks generated from serial sections have a high resolution, although the issues of section registration and distortion remain unsolved. Dec 17, 2001 episcopic fluorescence image capturing efic provides a simple and powerful tool for analyzing embryo and organ morphology in normal and transgenic embryos. Threedimensional reconstruction of rat knee joint using.
Asme 2012 summer bioengineering conference, parts a and b. Copy citation download citations reprints and permissions. This application seeks to acquire a confocal enhanced episcopic fluorescence image capture efic system. For samples suspected to have structural heart defects, the heartlung was paraffin embedded, and sectioned using an sm2500 sledge microtome and imaged using episcopic fluorescence image capture efic as previously described. The vertical illuminator in the center of the diagram has the light source at one end episcopic lamphouse and the filter cube at the other. Specimens are embedded in methacrylate resin and sectioned on a microtome.
This study aimed to analyze the spatial developmental changes of rat cruciate ligaments by threedimensional 3d reconstruction using episcopic fluorescence image capture efic. Clicking on the outside button will revert the image to an external view of the microscope and change the button to read inside. Automation of a relatively rapid image capture cycle and the ability to choose interimage distances as little as 1. Here, highresolution serial images are captured continuously from the block face instead of tissue sections. Methodsknee joints of wister rat embryos between embryonic day e14 and e20 were investigated. Please follow the links below to navigate to points of interest.
Highresolution episcopic microscopy hrem simple and. Episcopic fluorescence image capture efic was devised and developed in the early 2000s. Multifluorescence highresolution episcopic microscopy mfhrem. A remarkably simple solution has come about with the development of a new technique referred to as episcopic fluorescence image capture efic. Rapid acquisition of 3d images using highresolution. What is the abbreviation for episcopic fluorescence image. Spatial change of cruciate ligaments in rat embryo knee joint.
The 32bit version is available in the start menu as ic capture 2. Epi fluorescence, or incident light fluorescence, has now become the method of. Episcopic fluorescence image capture efic is widely applied for experimental animals we present a case of a patient whose l1cam gene in x. Fluorescence is the emission of light by a substance that has absorbed light or other electromagnetic radiation. Proceedings of the asme 2012 summer bioengineering conference. Episcopic fluorescence image capture efic and high resolution episcopic microscopy hrem are blockface imaging techniques that acquire superior resolution,1 mm of sample autofluorescence 8.
Weve got 1 shorthand for episcopic fluorescence image capture what is the abbreviation for episcopic fluorescence image capture. We have divided the fluorescence section of the primer into several categories to make it easier to organize and download. Fluorescence microscopy interactive tutorials reflected light fluorescence microscopy light pathways. Developmental atlas of the early first trimester human embryo. Apr 27, 2006 the branching of a large breast duct system was modelled using data extracted from cleared and stained 2 mm subgross sections of an autopsy breast using a publicdomain neuron modelling program cvapp, and episcopic fluorescence image capture efic was used to collect a stack of 1100 autofluorescence images of ducts in a mastectomy nipple. Episcopic fluorescence image capture efic and highresolution episcopic microscopy hrem are episcopic microscopy imaging methods 4. Pdf countryspecific mortality and growth failure in infancy and yound children and association with material stature use interactive graphics and. Cmos camera industrial camera microscope camera usb. Development of the knee joint was morphologically investigated, and the process of cavitation was analyzed by using episcopic fluorescence image captu. Using episcopic fluorescence image capture, ultrasound. Takakuwa y y human health science, graduate school of medicine, kyoto university, kyoto, japan.
Reflected light fluorescence microscopy light pathways. Sep 15, 2008 rosenthal j, mangal v, walker d, bennett m, mohun tj, lo cw. A detailed comparison of mouse and human cardiac development. Thermo fisher scientific offers a fluorescence imaging solution to fit your applicationyou choose the system that is right for you based on the imaging applications you want to run. Epifluorescence, or incident light fluorescence, has now become the method of choice in many applications and comprises a large part of this tutorial. Imaging heart development using highresolution episcopic. In our previous study reported by takaishi, 7 we showed the 3d structural development of the rat knee joint using episcopic fluorescence image capture efic. Development of the knee joint was morphologically investigated, and the process of cavitation was analyzed by using episcopic fluorescence image capture efic to create spatial and temporal threedimensional 3d reconstructions. Time course of atrial, ventricular, and outflow septation were. Similarly, episcopic fluorescence image capture efic, a novel histological imaging technique, provides registered 2dimensional 2d image stacks that can be resectioned in arbitrary planes and. Chd observed included outflow anomalies, leftright heart obstructive lesions, septalvalvular defects and cardiac situs anomalies.